Catalog Number:

RA18018

Product Type:

Affinity purified

Immunogen Sequence:

Synthetic phospho peptide surrounding Tyr490

Host:

Rabbit

Reactivity:

Human, Mouse and Rat

Applications:

Immunocytochemistry, Western blotting, Immunohistochemistry, Immunoprecipitation

Description:

TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk protoongogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

Reference:

Segal RA & Greenberg ME Intracellular signaling pathways activated by neurotrophic factors. Annu Rev Neurosci 19:463-89 (1996).

Stephens RM et al. Trk receptors use redundant signal transduction pathways involving SHC and PLC-gamma 1 to mediate NGF responses. Neuron 12:691-705 (1994). Lee CQ, Yun YD,.