Catalog Number:

RA18009

Product Type:

Affinity Purified

Immunogen Sequence:

Synthetic phospho peptide surrounding Ser9

Host:

Rabbit

Reactivity:

Human, Mouse and Rat

Applications:

Western blotting

Description:

Synapsin I is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal.

Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.

Reference:

Kao HT et al. A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nat Neurosci 5:431-7 (2002)

Jovanovic JN et al. Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-53 (2001)
Menegon A et al. Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals. J Cell Sci 113 ( Pt 20):3573-82 (2000)

Hosaka M et al. A phospho-switch controls the dynamic association of synapsins with synaptic vesicles. Neuron 24:377-87 (1999)