Catalog Number:

RA17100

Product Type:

Affinity Purified Antiserum

Immunogen Sequence:

WPPRPSPSAGLSLEA
a.a. 7-21 of the rat NTS2 receptor

Host:

Rabbit

Reactivity:

Rat

Applications:

Immunohistochemistry, Immunocytochemistry

Description:

The tridecapeptide neurotensin (NTS) is widely distributed in various regions of the brain and in peripheral tissues. Its effects are mediated by specific membrane receptors including neurotensin receptor-1 (NTSR1). NTSR2 shares 38% amino acid identity with human NTSR1 and over 75% amino acid identity with rat and mouse Ntsr2. NTSR2 contains a short cytoplasmic N-terminal sequence with no clear potential glycosylation sites. A cysteine residue is conserved in the human, mouse, and rat proteins in each of the extracellular loops between the 7 transmembrane domains. Threonine and serine residues that could be targets for phosphorylation are conserved in the human and rat proteins, as is a cysteine that could be a candidate for palmitoylation, thereby anchoring the region to the plasma membrane.

Reference:

Chalon, P.; Vita, N.; Kaghad, M.; Guillemot, M.; Bonnin, J.; Delpech, B.; Le Fur, G.; Ferrara, P.; Caput, D. : Molecular cloning of a levocabastine-sensitive neurotensin binding site. FEBS Lett. 386: 91-94, 1996.

Vita, N.; Oury-Donat, F.; Chalon, P.; Guillemot, M.; Kaghad, M.; Bachy, A.; Thurneyssen, O.; Garcia, S.; Poinot-Chazel, C.; Casellas, P.; Keane, P.; Le Fur, G.; Maffrand, J. P. : Soubrie, P.; Caput, D.; Ferrara, P.: Neurotensin is an antagonist of the human neurotensin NT2 receptor expressed in Chinese hamster ovary cells. Europ. J. Pharm. 360: 265-272, 1998.