Catalog Number:

RA15046

Product Type:

Affinity Purified Antibody

Immunogen Sequence:

CRGTELDCGIETD
a.a. 163 - 175 Caspase-3.

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

Immunocytochemistry, Western Blot

Description:

Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Reference:

Fernandes-Alnemri, T.; Armstrong, R. C.; Krebs, J.; Srinivasula, S. M.; Wang, L.; Bullrich, F.; Fritz, L. C.; Trapani, J. A.; Tomaselli, K. J.; Litwack, G.; Alnemri, E. S. : In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Nat. Acad. Sci. 93: 7464-7469, 1996.

Fernandes-Alnemri, T.; Litwack, G.; Alnemri, E. S. : CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269: 30761-30764, 1994.

Fernando, P.; Kelly, J. F.; Balazsi, K.; Slack, R. S.; Megeney, L. A. : Caspase 3 activity is required for skeletal muscle differentiation. Proc. Nat. Acad. Sci. 99: 11025-11030, 2002.