Catalog Number:

RA15043

Product Type:

Affinity Purified Antibody

Immunogen Sequence:

(C)ADLREDPDRQDHHPGSGAQ

Host:

Rabbit

Reactivity:

Human

Applications:

Immunohistochemistry, Western Blot

Description:

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer?s disease, Lewy bodies seen in Parkinson’s disease, and Pick bodies found in Pick’s disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Reference:

Perry G et al. Immunochemical properties of ubiquitin conjugates in the paired helical filaments of Alzheimer disease. J Neurochem 52:1523-8 (1989).

Perry G et al. Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains. Proc Natl Acad Sci U S A 84:3033-6 (1987).

Vierstra RD et al. Purification and initial characterization of ubiquitin from the higher plant, Avena sativa. J Biol Chem 260:12015-21 (1985).