Catalog Number:

RA15002

Product Type:

Affinity Purified Antibody

Immunogen Sequence:

synthetic phosphopeptide including residue T202/Y204 of extracellular signal-regulated kinase-1 (ERK1; MAPK3, p44 MAPK) and residues T185/Y187 of ERK2 (MAPK1, p42 MAPK)

Host:

Rabbit

Reactivity:

Human, Rat, Mouse

Applications:

Immunohistochemistry, Western Blotting

Description:

Epidermal growth factor induces immediate, ERK1/ERK2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/ERK2 causes an equally immediate reversion to the basal transcription level. ERK1/ERK2 was found to phosphorylate the architectural transcription factor UBF at amino acids 117 and 201 within HMG boxes 1 and 2, preventing their interaction with DNA. Mutation of these sites inhibited transcription activation and abrogated the transcriptional response to ERK1/ERK2. Thus, growth factor regulation of ribosomal transcription likely acts by a cyclic modulation of DNA architecture. The data suggested a central role for ribosome biogenesis in growth regulation

Reference:

Agrawal, A.; Lingappa, J.; Leppla, S. H.; Agrawal, S.; Jabbar, A.; Quinn, C.; Pulendran, B. :
Impairment of dendritic cells and adaptive immunity by anthrax lethal toxin. Nature 424: 329-334, 2003.

Charest, D. L.; Mordret, G.; Harder, K. W.; Jirik, F.; Pelech, S. L. :
Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1. Molec. Cell. Biol. 13: 4679-4690, 1993.

Di Cristo, G.; Berardi, N.; Cancedda, L.; Pizzorusso, T.; Putignano, E.; Ratto, G. M.; Maffei, L. :
Requirement of ERK activation for visual cortical plasticity. Science 292: 2337-2340, 2001.