Catalog Number:

RA10106

Product Type:

Polyclonal Antiserum

Immunogen Sequence:

FGGFTGARKSARKLANQ - Corresponding to residues 122-138 of rar Orphanin FQ

Host:

Rabbit

Reactivity:

Rat, Primate

Applications:

Immunohistochemistry 1:500

Description:

Orphanin FQ (nociceptin, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of OFQ hashomology
with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for OFQ in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated OFQ abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. OFQ immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Reference:

Meunier, J. C., Mollereau, C., Toll, L., Suaudeau, C., Moisand, C., Alvinerie, P., Butour, J. L., Guillemot, J. C., Ferrara, P., Monsarrat, B., and et al. (1995). Isolation and structure of the endogenous agonist of opioid receptor- like ORL1 receptor. Nature 377, 532-5.

Reinscheid, R. K., Nothacker, H. P., Bourson, A., Ardati, A., Henningsen, R. A, Bunzow, J. R., Grandy, D. K., Langen, H., Monsma, F. J., Jr., and Civelli, O. (1995). Orphanin FQ: a neuropeptide that activates an opioidlike G proteincoupled receptor. Science 270, 792-4.

Riedl, M., Shuster, S., Vulchanova, L., Wang, J., Loh, H. H., and Elde, R. (1996). Orphanin FQ/nociceptin-immunoreactive nerve fibers parallel those containing endogenous opioids in rat spinal cord. Neuroreport 7, 1369-72.