Catalog Number:

MO19001

Product Type:

Affinity Purified

Immunogen Sequence:

Glial fibrillary protein from porcine spinal cord

Host:

Mouse

Reactivity:

Human, pig, mouse and rat

Applications:

Western Blot, Immunohistochemistry on paraffin sections

Description:

Glial fibrillary acidic protein is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage. The predicted amino acid sequence indicates that GFAP shares structural similarities--particularly in the central rod domain and to a lesser degree in the carboxyl-terminal domain--with other IF proteins found in nonepithelial cell types. Considerable sequence divergence in the amino-terminal region of GFAP suggested that the tissue-specific functions of this IF protein may be mediated through this region of the molecule. GFAP is a useful marker of astroglia in the brain.

Reference:

Liedtke, W.; Edelmann, W.; Bieri, P. L.; Chiu, F.-C.; Cowan, N. J.; Kucherlapati, R.; Raine, C. S. : GFAP is necessary for the integrity of CNS white matter architecture and long-term maintenance of myelination. Neuron 17: 607-615, 1996

Brownell, E.; Lee, A. S.; Pekar, S. K.; Pravtcheva, D.; Ruddle, F. H.; Bayney, R. M.: Glial fibrillary acid protein, an astrocytic-specific marker, maps to human chromosome 17. Genomics 10: 1087-1089, 1991

Bongcam-Rudloff, E.; Nister, M.; Betsholtz, C.; Wang, J.-L.; Stenman, G.; Huebner, K.; Croce, C. M.; Westermark, B.: Human glial fibrillary acidic protein: complementary DNA cloning, chromosome localization, and messenger RNA expression in human glioma cell lines of various phenotypes. Cancer Res. 51: 1553-1560, 1991