Catalog Number:

MO19000

Product Type:

Purified

Immunogen Sequence:

Purified Calbindin-D from chicken gut

Host:

Mouse

Reactivity:

Human, monkey, rabbit, rat, mouse,
chicken, hamster, sheep, guinea pig and
fish

Applications:

Immunohistochemistry on paraffin sections, Western Blot

Description:

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Calbindin immunoreactivity was further detected by radioimmunoassay and immunohistochemistry in the kidney, pancreatic islets, and brain. In the brain, its synthesis is independent of vitamin-D-derived hormones. Two different proteins presenting calbindin immunoreactivity, one of molecular mass 27 kD (now known to be 28 kD) and the other of 29 kD, were identified in the central nervous system. Both molecular species are present in the brain of all vertebrates except fish. Parmentier et al. (1987) selected human 28-kD calbindin cDNA clones by antibody screening of lambda-gt11 brain libraries. The sequence showed an open reading frame coding for a protein of 261 amino acids, containing 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The preliminary data suggested that the 29-kD protein in brain is encoded by a different gene. By means of immunohistochemical methods, Seto-Ohshima et al. (1988) demonstrated a dearth of neurons containing calbindin in the brains of patients with Huntington disease. Calbindin depletion was particularly notable in the neostriatum (caudate nucleus and putamen) of these patients.

Reference:

Parmentier, M.; et al.: The human calbindin 27 kDa gene: structural organization of the 5-prime and 3-prime regions, chromosomal assignment and restriction fragment length polymorphism. Genomics 4: 309-319, 1989.

Parmentier, M.; et al.: Human 27-kDa calbindin complementary DNA sequence: evolutionary and functional implications. Europ. J. Biochem. 170: 207-215, 1987.

Parmentier, M.; et al.: The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3-q22.1 and 16q22-q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci. Cytogenet. Cell Genet. 57: 41-43, 1991.

Seto-Ohshima, A.; et al.: Loss of matrix calcium-binding protein-containing neurons in Huntington's disease. Lancet I: 1252-1254, 1988.