Catalog Number:

MO18001

Product Type:

Affinity Purified

Immunogen Sequence:

Recombinant rat tumor necrosis factor alpha

Host:

Goat

Reactivity:

Rat

Applications:

Immunohistochemistry on fixed sections, ELISA, Western Blot

Description:

Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. The C-terminus of ubiquitin forms an isopeptide bond with the amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. One frequent target of the ubiquitin modification machinery is transcription factors. Although ubiquitination generally modulates their function by inducing proteasome-dependent degradation, past and recent studies indicate that ubiquitination also regulates nuclear-cytoplasmic trafficking of transcriptional regulators. Ubiquitination is known to modulate transcription factor localization by destroying sequestering proteins and by directly promoting nuclear import and export of modified substrates.

Reference:

Shcherbik N, Haines DS. Ub on the move J Cell Biochem. 2004 Sep 1;93(1):11-9

Hegde AN. Ubiquitin-proteasome-mediated local protein degradation and synaptic plasticity. Prog Neurobiol. 2004 Aug;73(5):311-57