Catalog Number:

MO15023

Product Type:

Purified

Immunogen Sequence:

Recombinant mouse Semaphorin 6A extracellular domain

Host:

Rat IgG2a

Reactivity:

Mouse

Applications:

Immunohistochemistry on frozen sections, ELISA, Western Blot

Description:

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. The human SEMA6A gene encodes a protein of 1,030 amino acids with a calculated molecular mass of 112.2 kD. Highest expression was observed in embryonic brain and kidney, whereas only low to moderate expression was seen in developing lung and liver. Only small amounts of SEMA6A transcripts were detected in human adult tissues, with the exception of rather strong expression levels in the highly regenerative placental tissues. SEMA6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. However, the length of the cytoplasmic tail, which includes an EVL-binding site in SEMA6A and an Src-binding site in SEMA6B, suggests that these semaphorins may also function as receptors.

Reference:

Klostermann, A.; Lutz, B.; Gertler, F.; Behl, C. : The orthologous human and murine semaphorin 6A-1 proteins (SEMA6A-1/Sema6A-1) bind to the enabled/vasodilator-stimulated phosphoprotein-like protein (EVL) via a novel carboxyl-terminal zyxin-like domain. J. Biol. Chem. 275: 39647-39653, 2000.

Leighton, P. A.; Mitchell, K. J.; Goodrich, L. V.; Lu, X.; Pinson, K.; Scherz, P.; Skarnes, W. C.; Tessier-Lavigne, M. : Defining brain wiring patterns and mechanisms through gene trapping in mice. Nature 410: 174-179, 2001