Catalog Number:

MO15022

Product Type:

Protein G Purified

Immunogen Sequence:

Mouse inoculated with hCXCR4 transfected mouse 3T3 cells

Host:

Mouse

Reactivity:

Human, Cat

Applications:

Immunohistochemistry on frozen sections, Immunocytochemistry, FACS

Description:

CXCR4 (old designation: LESTR , HUMSTR). has been identified also asNPYRL (neuropeptide Y receptor-like) and HM89 . CXCR4 also known as fusin acts as one of the receptors for CXC-Chemokines . Two variants of the receptor, designated CXCR4A and CXCR4B , have been described. Expression of CXCR3 and CXCR4 on human NK-cells is up-regulated by TGF-beta-1 , but not by IFN-gamma , MIP-3-beta , MDC , TARC or I-309. The new designation for this receptor is CD184. CXCR4 has been shown to be a cofactor required for entry of HIV into cells expressing CD4 . It functions as an inhibitor of infection by T-cell-tropic HIV-1 strains. During the early stages of HIV infection, viral isolates tend to use the chemokine receptor CCR5 for viral entry, while later isolates tend to use CXCR4. Both splice variants of CXCR4 have been shown to function as receptors for SDF-1 . In cell lines expressing CXCR4 and CD4 , and in blood lymphocytes, SDF-1 is a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.
Human CXCR4 exists in antigenically distinct conformations on many cell lines and primary cell types. As a result, to efficiently detect cell surface CXCR4, an antibody that recognizes multiple CXCR4 conformations is desirable. The customer should consult the publication by Baribaud, et al., 2001 J. Virol. 75:8957 as a guide for the optimal selection of monoclonals. Monoclonal 44716 has been shown to recognize multiple CXCR4 conformations. Use of other monoclonals may result in an underestimation of the density of CXCR4 expression on certain cell types. The above reference also contains epitope mapping details for many CXCR4 monoclonals.

Reference:

1. Wegner SA et al Genomic organization and functional characterization of the chemokine receptor CXCR4, a major entry co-receptor for human immunodeficiency virus type 1. Journal of Biological Chemistry 273(8): 4754-4760 (1998)

Oberlin E et al The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1 Nature (London) 382(6594): 833-835 (1996).

Bleul CC et al The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes. Proceedings of the National Academy of Science (USA) 94(5): 1925-1930 (1997).