Catalog Number:

MO15011

Product Type:

Mouse monoclonal IgG3

Immunogen Sequence:

Human APJ transfected cells

Host:

Mouse

Reactivity:

Human

Applications:

Immunohistochemistry

Description:

Bioassays with human APJ-expressing CHO cells confirmed the presence of active apelin in rat mammary gland, rat milk, and at variable levels in bovine milk, with highest concentrations in colostrum.

Tatemoto et al. (1998) purified a protein that bound to the APJ receptor. Based on peptide sequences, they cloned the corresponding human cDNA. The gene encodes a 77-amino acid polypeptide that includes a secretory signal sequence. Synthetic 36-amino acid apelin (apelin-36), corresponding to the most C-terminal 36 residues of preproapelin, caused the acidification of cells expressing transfected APJ, but not other receptors. The authors stated that apelin is an endogenous ligand for the APJ receptor

Reference:

Cayabyab, M.; Hinuma, S.; Farzan, M.; Choe, H.; Fukusumi, S.; Kitada, C.; Nishizawa, N.; Hosoya, M.; Nishimura, O.; Messele, T.; Pollakis, G.; Goudsmit, J.; Fujino, M.; Sodroski, J. : Apelin, the natural ligand of the orphan seven-transmembrane receptor APJ, inhibits human immunodeficiency virus type 1 entry. J. Virol. 74: 11972-11976, 2000.

Habata, Y.; Fujii, R.; Hosoya, M.; Fukusumi, S.; Kawamata, Y.; Hinuma, S.; Kitada, C.; Nishizawa, N.; Murosaki, S.; Kurokawa, T.; Onda, H.; Tatemoto, K.; Fujino, M. : Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum. Biochim. Biophys. Acta 1452: 25-35, 1999.

Scott, A. F. Personal Communication. Baltimore, Md., 2/7/2001. 4.

Tatemoto, K.; Hosoya, M.; Habata, Y.; Fujii, R.; Kakegawa, T.; Zou, M.-X.; Kawamata, Y.; Fukusumi, S.; Hinuma, S.; Kitada, C.; Kurokawa, T.; Onda, H.; Fujino, M. : Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochem. Biophys. Res. Commun. 251: 471-476, 1998.