Catalog Number:

GT15080

Product Type:

Affinity Purified

Immunogen Sequence:

NS0-derived rmTrkB extracellular domain

Host:

Goat

Reactivity:

Mouse

Applications:

Immunohistochemsitry on frozen sections, Western Blotting, ELISA

Description:

The Trk proto-oncogene encodes a tyrosine protein kinase, Trk gp140, also designated Trk A, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) (1–3) and neurotrophin-3 (NT-3) (4). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems (5,6). The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B (5,6), although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a 140 kDa protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for neurotrophin-3 (NT-3) (7).

Reference:

1. Hempstead, B.L., Martin-Zanca, D., Kaplan, D.R., Parada, L.F., and Chao, M.V. 1991. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the lowaffinity NGF receptor. Nature 350: 678-683.

2. Kaplan, D.R., Martin-Zanca, D., and Parada, L.F. 1991. Tyrosine phosphorylation and tyrosine kinase activity of the trk proto-oncogene product induced by NGF. Nature 350: 158-160.

3. Klein, R., Jing, S., Nanduri, V., O’Rourke, E., and Barbacid, M. 1991. The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 65: 189-197.

4. Cordon-Cardo, C., Tapley, P., Jing, S., Nanduri, V., O’Rourke, E., Lamballe, F., Kovary, K., Klein, R., Jones, K.R., Reichardt, L.F., and Barbacid, M. 1991. The trk tyrosine protein kinase mediates the mitogenic properties of nerve growth factor and neurotrophin-3. Cell 66: 173-183.

5. Klein, R., Nanduri, V., Jing, S., Lamballe, F., Tapley, P., Bryant, S., Cordon-Cardo, C., Jones, K.R., Reichardt, L.F., Barbacid, M. 1991. The trk B tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. Cell 66: 395-403.

6. Klein, R., Parada, L.F., Coulier, F., and Barbacid, M. 1989. trk B, a novel tyrosine protein kinase receptor expressed during mouse neural development. EMBO J. 8: 3701-3709.

7. Lamballe, F., Klein, R., and Barbacid, M. 1991. trk C, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin-3. Cell 66: 967-979.

8. Klein, R., Conway, D., Parada, L.F., and Barbacid, M. 1990. The trk B tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell 61: 647-656.