Catalog Number:

GT15076

Product Type:

Affinity purified

Immunogen Sequence:

NS0-derived rmSema 7a

Host:

Goat

Reactivity:

Mouse

Applications:

Western Blotting, ELISA

Description:

Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences, and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. SEMA7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. SEMA7A is also known as the John-Milton-Hagen (JMH) blood group antigen, an 80-kD glycoprotein expressed on activated lymphocytes and erythrocytes. It was shown that semaphorin 7A can beside its immunomodulatory effects also mediate neuronal functions. Unlike many other semaphorins, which act as repulsive guidance cues, SEMA7A enhances central and peripheral axon growth and is required for proper axon tract formation during embryonic development.

Reference:

1. Lange, C.; Liehr, T.; Goen, M.; Gebhart, E.; Fleckenstein, B.; Ensser, A. : New eukaryotic semaphorins with close homology to semaphorins of DNA viruses. Genomics 51: 340-350, 1998.

2. Pasterkamp, R. J.; Peschon, J. J.; Spriggs, M. K.; Kolodkin, A. L. : Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature 424: 398-405, 2003.

3. Sato, Y.; Takahashi, H. : Molecular cloning and expression of murine homologue of semaphorin K1 gene. Biochim. Biophys. Acta 1443: 419-422, 1998.

4. Xu, X.; Ng, S.; Wu, Z.-L.; Nguyen, D.; Homburger, S.; Seidel-Dugan, C.; Ebens, A.; Luo, Y. : Human semaphorin K1 is glycosylphosphatidylinositol-linked and defines a new subfamily of viral-related semaphorins. J. Biol. Chem. 273: 22428-22434, 1998.

5. Yamada, A.; Kubo, K.; Takeshita, T.; Harashima, N.; Kawano, K.; Mine, T.; Sagawa, K.; Sugamura, K.; Itoh, K. : Molecular cloning of a glycosylphosphatidylinositol-anchored molecule CDw108. J. Immun. 162: 4094-4100, 1999.