Catalog Number:

GT15067

Product Type:

Affinity Purified

Immunogen Sequence:

Synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human c-Jun

Host:

Goat

Reactivity:

Mouse

Applications:

ELISA, Western Blot, Immunohistochemistry on frozen sections, immunocytochemistry

Description:

The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. Mammalian hedgehog genes share striking homology to the Drosophila segment polarity gene hedgehog, a key regulator of pattern formation in the embryonic and adult fly. The human DHH gene encodes a 396-amino acid polypeptide. Bitgood and McMahon (1995) and Parmantier et al. (1999) showed that during development in the mouse, Dhh mRNA shows a very restricted distribution, being expressed primarily in Sertoli cells of developing testes and in Schwann cells of peripheral nerves. It was shown that Dhh intercellular signal is essential for a variety of patterning events during development. It may function as a spermatocyte survival factor in the testes and is essential for testes development. The C-terminal peptide diffuses from the cell, while the N-terminal peptide remains associated with the cell surface. Is also secreted in either cleaved or uncleaved form to mediate signaling to other cells. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Reference:

Bitgood, M. J.; McMahon, A. P. : Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo. Dev. Biol. 172: 126-138, 1995.

Parmantier, E.; Lynn, B.; Lawson, D.; Turmaine, M.; Namini, S. S.; Chakrabarti, L.; McMahon, A. P.; Jessen, K. R.; Mirsky, R.: Schwann cell-derived desert hedgehog controls the development of peripheral nerve sheath. Neuron 23: 713-724, 1999

Marigo, V.; Roberts, D. J.; Lee, S. M. K.; Tsukurov, O.; Levi. T.; Gastier, J. M.; Epstein, D. J.; Gilbert, D. J.; Copeland, N. G.; Seidman, C. E.; Jenkins, N. A.; Seidman, J. G.; McMahon, A. P.; Tabin, C. Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene Hedgehog. Genomics 28: 44-51, 1995