Catalog Number:

GT15064

Product Type:

Affinity Purified

Immunogen Sequence:

Recombinant rat tumor necrosis factor alpha

Host:

Goat

Reactivity:

Rat

Applications:

Immunohistochemistry on fixed sections, ELISA, Western Blot

Description:

TNF alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin 1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation. TNF alpha is a type II membrane protein, but also exists as an extracellular soluble form. The soluble form derives from the membrane form by proteolytic processing.

Reference:

Bouwmeester, T.; Bauch, A.; Ruffner, H.; Angrand, P.-O.; Bergamini, G.; Croughton, K.; Cruciat, C.; Eberhard, D.; Gagneur, J.; Ghidelli, S.; Hopf, C.; Huhse, B.; and 16 others : A physical and functional map of the human TNF-alpha/NF-kappa-B signal transduction pathway. Nature Cell Biol. 6: 97-105, 2004

Nedwin, G. E.; Naylor, S. L.; Sakaguchi, A. Y.; Smith, D.; Jarrett-Nedwin, J.; Pennica, D.; Goeddel, D. V.; Gray, P. W. : Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 13: 6361-6373, 1985.

Old, L. J. : Tumor necrosis factor (TNF). Science 230: 630-632, 1985.