Catalog Number:

GT15052

Product Type:

Affinity Purified

Immunogen Sequence:

recombinant human Oct 3/4

Host:

Goat

Reactivity:

Human, mouse

Applications:

Immunocytochemistry, Western Blot, ELISA

Description:

Niwa et al. (2000) used conditional expression and repression in murine embryonic stem (ES) cells to determine requirements for Oct3/4 in the maintenance of developmental potency. Although transcriptional determination has usually been considered as a binary on-off control system, they found that the precise level of Oct3/4 governs 3 distinct fates of ES cells. A less-than-2-fold increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus, a critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. Niwa et al. (2000) suggested that their findings established a role for Oct3/4 as a master regulator of pluripotency that controls lineage commitment and illustrated the sophistication of critical transcriptional regulators and the consequent importance of quantitative analyses

Reference:

Niwa, H.; Miyazaki, J.; Smith, A. G. : Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genet. 24: 372-376, 2000.

Rosner, M. H.; De Santo, R. J.; Arnheiter, H.; Staudt, L. M. : Oct-3 is a maternal factor required for the first mouse embryonic division. Cell 64: 1103-1110, 1991. Note: Retraction: Cell 69: 724 only, 1992.