Catalog Number:

GT15045

Product Type:

Affinity purified

Immunogen Sequence:

Recombinant human
Caspase-9, aa 139 - 330

Host:

Goat

Reactivity:

Human

Applications:

Immuncytochemistry, Immunoprecipitation, Western Blot

Description:

Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Reference:

Hakem, R.; Hakem, A.; Duncan, G. S.; Henderson, J. T.; Woo, M.; Soengas, M. S.; Elia, A.; de la Pompa, J. L.; Kagi, D.; Khoo, W.; Potter, J.; Yoshida, R.; Kaufman, S. A.; Lowe, S. W.; Penninger, J. M.; Mak, T. W. : Differential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94: 339-352, 1998.

Shiozaki, E. N.; Chai, J.; Rigotti, D. J.; Riedl, S. J.; Li, P.; Srinivasula, S. M.; Alnemri, E. S.; Fairman, R.; Shi, Y. : Mechanism of XIAP-mediated inhibition of caspase-9. Molec. Cell 11: 519-527, 2003.