Catalog Number:

GT15026

Product Type:

Affinity Purified

Immunogen Sequence:

Recombinant mouse Ephrin-B2
extracellular domain

Host:

Goat

Reactivity:

Mouse, Rat

Applications:

Immunohistochemistry, Western Blot, ELISA

Description:

By treating embryonic rat cortical neurons with Efnb2, followed by stimulation with glutamate, Takasu et al. (2002) observed a large increase in intracellular calcium that was dependent on the cytoplasmic domain of the ephrin receptor Ephb2 (600997). Treatment with Bdnf did not result in an increase in glutamate-stimulated intracellular calcium. Western blot analysis showed that Efnb2 treatment increased tyrosine phosphorylation of NMDA receptor 2B (Nr2b, or Grin2b; 138252) at positions 1252, 1336, and 1472 by the Src family tyrosine kinase Fyn (137025). Efnb2 treatment also increased phosphorylation of Creb (123810) at ser133, which was mediated by the NMDA receptor. In addition, Efnb2 treatment potentiated glutamate activation of Bdnf and Cpg15. Takasu et al. (2002) concluded that the EFNB-EPHB-NMDA receptor interaction may represent an early step in the initiation of synapse formation or maturation and may potentiate the ability of the NMDA receptor to respond to activity-dependent signals from the extracellular milieu. In a perspective, Ghosh (2002) noted that Grunwald et al. (2001) and Henderson et al. (2001) reported that mice lacking Ephb2 had defective synaptic plasticity, particularly at CA1 synapses, possibly due to a lack of proper clustering of NMDA receptors at the synapse.

Reference:

Adams, R. H.; Diella, F.; Hennig, S.; Helmbacher, F.; Deutsch, U.; Klein, R. :
The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration. Cell 104: 57-69, 2001.

Bennett, B. D.; Zeigler, F. C.; Gu, Q.; Fendly, B.; Goddard, A. D.; Gillett, N.; Matthews, W. :
Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk. Proc. Nat. Acad. Sci. 92: 1866-1870, 1995.

Bonaldo, M. F.; Yu, M. T.; Jelenc, P.; Brown, S.; Su, L.; Lawton, L.; Deaven, L.; Efstratiadis, A.; Warburton, D.; Soares, M. B. :
Selection of cDNAs using chromosome-specific genomic clones: application to human chromosome 13. Hum. Molec. Genet. 3: 1663-1673, 1994.