Catalog Number:

GT15020

Product Type:

Affinity Purified

Immunogen Sequence:

Recombinant mouse matrix
metalloproteinase 9

Host:

Goat

Reactivity:

Mouse, Rat

Applications:

Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA

Description:

Interleukin-8 (IL8) induces rapid mobilization of hematopoietic progenitor cells (HPCs) from the bone marrow of rhesus monkeys. Because activation of neutrophils by IL8 induces the release of MMP9, which is involved in the degradation of extracellular matrix molecules. MMP9 release might induce stem cell mobilization by cleaving matrix molecules to which stem cells are attached. Mobilization of HPCs could be prevented by pretreatment with an inhibitory anti-gelatinase B antibody, indicating that MMP9 is involved as a mediator of the IL8-induced mobilization of HPCs. MMP9-mediated N-terminal cleavage of IL8 potentiates IL8 activation of neutrophils, as measured by increased intracellular calcium, MMP9 secretion, and neutrophil chemotaxis.

Reference:

Collier, I. E.; Bruns, G. A. P.; Goldberg, G. I.; Gerhard, D. S. :
On the structure and chromosome location of the 72- and 92-kDa human type IV collagenase genes. Genomics 9: 429-434, 1991.

Coussens, L. M.; Tinkle, C. L.; Hanahan, D.; Werb, Z. :
MMP-9 supplied by bone marrow-derived cells contributes to skin carcinogenesis. Cell 103: 481-490, 2000.