Catalog Number:

GP14020

Product Type:

Serum

Immunogen Sequence:

TVGTHNGLEHSTFNMTIEPGRVQAYC

Host:

Guinea Pig

Reactivity:

Rat

Applications:

Immunocytochemistry

Description:

The tridecapeptide neurotensin is widely distributed in various regions of the brain and in peripheral tissues. In the brain, neurotensin acts as a neuromodulator, in particular of dopamine transmission in the nigrostriatal and mesocorticolimbic systems, suggesting its possible implication in dopamine-associated behavioral neurodegenerative and neuropsychiatric disorders. Its various effects are mediated by specific membrane receptors. Vita et al. (1993) isolated a cDNA encoding a human neurotensin receptor (NTSR1) and showed that it predicts a 418-amino acid protein that shares 84% homology with the rat protein. Le et al. (1997) also cloned human NTSR1 cDNA and its genomic DNA. The gene contains 4 exons and spans more than 10 kb. The authors identified a highly polymorphic tetranucleotide repeat approximately 3 kb from the gene. Southern blot analysis revealed that the NTSR1 gene is present in the human genome as a single-copy gene. Le et al. (1997) stated that the neurotensin receptor has 7 transmembrane spanning regions and high homology to other receptors that couple to G proteins.

Reference:

Laurent, P.; Clerc, P.; Mattei, M.-G.; Forgez, P.; Dumont, X.; Ferrara, P.; Caput, D.; Rostene, W. : Chromosomal localization of mouse and human neurotensin receptor genes. Mammalian Genome 5: 303-306, 1994.

Le, F.; Groshan, K.; Zeng, X. P.; Richelson, E. : Characterization of the genomic structure, promoter region, and a tetranucleotide repeat polymorphism of the human neurotensin receptor gene. J. Biol. Chem. 272: 1315-1322, 1997.

Vincent, J.-P. : Neurotensin receptors: binding properties, transduction pathways, and structure. Cell. Molec. Neurobiol. 15: 501-512, 1995.

Vita, N.; Laurent, P.; Lefort, S.; Chalon, P.; Dumont, X.; Kaghad, M.; Gully, D.; Le Fur, G.; Ferrara, P.; Caput, D. : Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor. FEBS Lett. 317: 139-142, 1993.